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Teach Soap • View topic - testing lotion for bacteria growth

Teach Soap

Soap Making Recipes, Tips and Tutorials
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PostPosted: Fri Jun 13, 2014 5:25 pm 
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is there a wat to test for bacterial growth at home? I took a biology course this year so I had to get a microscope(goes to 400x) so I was wondering if a way to test at home, I don't know if the bacteria would be visible on just a basic slide or not. I have no idea if anyone even knows the answer but it doesn't hurt to ask! :D

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PostPosted: Fri Jun 13, 2014 6:35 pm 
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This is the only one that I know of. http://www.lotioncrafter.com/microbial- ... after.html

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Irena
Closed minds are like faulty parachutes; they refuse to open.


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PostPosted: Fri Jun 13, 2014 6:39 pm 
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I want to do this, too! We also have a microscope (used it for biology course as well!), with which I want to look at a sample of a scrub I made to see if I can see anything bacteria-related. I'm assuming I need to look for movement (indicating something alive), but I don't know if it requires a stain or anything?

I bet it would work, though. I have experimented with bacterial cultures before (pond water sealed in a jar with boiled eggs, hay, or some other food source, sitting outside for a week or so; the stench was TERRIBLE when I opened it :shock: ). I simply used a dropper to put some of the pond water near the bottom of the jar on a slide and examined under the microscope; saw plenty of little wriggling organisms, no stain required. If it works with pond water, it will probably work with lotion or a scrub. At least, I am willing to try it!

I guess biology course paid off after all... :lol:

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PostPosted: Fri Jun 13, 2014 7:04 pm 
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PostPosted: Mon Jun 16, 2014 12:43 pm 
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That sounds like a good sign, Rosie!

I looked at some slides of my scrub under the microscope, and it looked OK to me! There was nothing alive at the magnification I can do with my microscope, so that is a good sign! However, mold and yeast, etc., don't move, so I don't know if there was any of that in there or not. Of course, I'm no expert, so I could have missed something important, but I did mix up a brand new scrub on the spot and observe it under the scope, too. It looked very similar to the older one. I think I am safe, considering bacteria don't grow very well in oil (correct?). But what had me worried was, I was afraid some water from the shower would get into the scrub, and then bacteria would grow in the water. And THEN, I saw a dark-looking cloud floating in the oil at the top of the scrub, and was deathly afraid it was a bacteria cloud or something. And while I could see the dark cloud under the microscope, it simply looked like a dark splotch, nothing deadly. I am now thinking it was just some vanilla extract that had separated out from the oils... :roll: Maybe I am paranoid, I just don't want to put bacteria-infested scrub all over me!

Would the lotion tester that you mentioned, soapbuddy, work for scrubs as well, or only lotion?

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PostPosted: Tue Jun 17, 2014 10:09 am 
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PostPosted: Thu Jun 19, 2014 6:24 am 

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biggest things are ensure you are using the correct percentage AND ensure you are really mixing good for excellent dispersion. If you only half heartedly mix, you may have "un-preserved" parts. Here andthere I will leave a sample of lotion of after shave balm out in the open for a week or so to see how it reacts.
Steve @ cardinham


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